The story of the film focused on the exceptional military unit known as GI Joe who was now on the task taking down an evil organization who aims for world domination and destruction of those who oppose.
The characters in the film were portrayed by Channing Tatum as "Duke" (Conrad Hauser), Dennis Quaid as "General Hawk" (General Clayton Abernathy), Marlon Wayans as "Ripcord" (Wallace Weems), Rachel Nichols as "Scarlett" (Shana M. O'Hara), Adewale Akinnuoye-Agbaje as "Heavy Duty" (Hershel Dalton), Ray Park as "Snake-Eyes", Saïd Taghmaoui as "Breaker" (Abel Shaz), Karolína Kurková as "Cover Girl" (Courtney Kreiger), and Brendan Fraser as "Sgt. Stone" (Geoffrey Stone IV). While on the antagonists part were portrayed by Joseph Gordon-Levitt as Cobra Commander (Rex Lewis/The Doctor) as the lead role followed by Christopher Eccleston as Destro (James McCullen XXIV), Lee Byung-hun as Storm Shadow (Thomas Arashikage), and Sienna Miller as The Baroness (Ana Lewis/Anastascia DeCobray).